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Extended spectrum betalactamase

1.Write a paragraph on ESBL ( Extended spectrum betalactamase). Please address the following points.

Definition ( type of antibiotics for which the organism/s are resistant)
Bacteria carrying ESBL gene
The magnitude of the problem in the US ( disease and mortality)
Genetic mechanism ( Chromosomal vs plasmid mediated)
How the resistant gene is acquired ( mutation or specific horizontal gene transfer).

2. Write a one paragraph summary for each topic below with a particular focus on medical application of the method or tool.
Restriction enzyme
Polymerase Chain reaction (PCR)

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