Data Sheet: Activity – Biomolecules
Procedure I – Group 1: Known Sample Solutions
Complete the table below using your data from Procedure I. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Fill in the last column (Biomolecule Classification) using data found under the Activity Form tab.
Data Table I
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Sample Solution
Test
Solution
1
Reaction
Test
Solution
2
Reaction
Test
Solution
3
Reaction
Test
Solution
4
Reaction
Test
Solution
5
Reaction
Biomolecule
Classification
A
Red
No change
No change
No change
No change
Sugar
B
Red
Purple
No change
No change
No change
Starch
C
No change
No
Change
Green
No change
No change
Fat
D
No change
No change
No change
Pink
No change
Protein
E
No change
No change
No change
Pink
Gray
Amino Acid
Observations and Questions
[1] Write a hypothesis statement for the experiment you conducted for Procedure I. [2] Which variable represents the independent variable? What information helped you to come to that decision? [3] Would you characterize this experiment as qualitative or quantitative? What information helped you to come to that decision? [4] Refer to your completed Data Table I and place the appropriate answer to each of the statements below.Answers: amino acid, starch, fat, sugar or starch, protein or amino acid
Statements:
Test Solution 1 reacts with:
Test Solution 2 reacts with:
Test Solution 3 reacts with:
Test Solution 4 reacts with:
Test Solution 5 reacts with:
Procedure II – Group 2: Unknown Sample Solutions
Complete the table below using your data from Procedure II. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Next, use Data Table I to determine the biomolecule classification for each sample solution. Hint: compare the color changes in both tables to make your determinations.
Data Table II
pg. 2
pg. 2
Sample Solution
Test
Solution
1
Reaction
Test
Solution
2
Reaction
Test
Solution
3
Reaction
Test
Solution
4
Reaction
Test
Solution
5
Reaction
Biomolecule
Classification
A
No change
No change
Green
Yellow
No change
Sugar
B
No change
No change
No change
Pink
Gray
Starch
C
Pink
Purple
No change
Yellow
No change
Fat
D
No change
No change
No change
Pink
No change
Protein
E
Pink
No change
No change
Yellow
No change
Amino Acid
Observations and Questions
[5] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain sugars? If yes, which one? What information helped you to come to that decision? [6] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain protein? If yes, which one? What information helped you to come to that decision? [7] Using the laboratory tests in this experiment, would you be able to distinguish between a small molecule such as amino acid from a larger molecule such as a protein? Explain why or why not. [8] Using the laboratory tests in this experiment, would you be able to distinguish between a starch and a sugar such as glucose? Explain why or why not.