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Assignment Paper

Data Sheet: Activity – Biomolecules
Procedure I – Group 1: Known Sample Solutions
Complete the table below using your data from Procedure I. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Fill in the last column (Biomolecule Classification) using data found under the Activity Form tab.

Data Table I

4

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2

Sample Solution

Test

Solution

1

Reaction

Test

Solution

2

Reaction

Test

Solution

3

Reaction

Test

Solution

4

Reaction

Test

Solution

5

Reaction

Biomolecule

Classification

A

Red

No change

No change

No change

No change

Sugar

B

Red

Purple

No change

No change

No change

Starch

C

No change

No

Change

Green

No change

No change

Fat

D

No change

No change

No change

Pink

No change

Protein

E

No change

No change

No change

Pink

Gray

Amino Acid

Observations and Questions

[1] Write a hypothesis statement for the experiment you conducted for Procedure I. [2] Which variable represents the independent variable? What information helped you to come to that decision? [3] Would you characterize this experiment as qualitative or quantitative? What information helped you to come to that decision? [4] Refer to your completed Data Table I and place the appropriate answer to each of the statements below.

Answers: amino acid, starch, fat, sugar or starch, protein or amino acid

Statements:

Test Solution 1 reacts with:

Test Solution 2 reacts with:

Test Solution 3 reacts with:

Test Solution 4 reacts with:

Test Solution 5 reacts with:

Procedure II – Group 2: Unknown Sample Solutions
Complete the table below using your data from Procedure II. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Next, use Data Table I to determine the biomolecule classification for each sample solution. Hint: compare the color changes in both tables to make your determinations.

Data Table II

pg. 2

pg. 2

Sample Solution

Test

Solution

1

Reaction

Test

Solution

2

Reaction

Test

Solution

3

Reaction

Test

Solution

4

Reaction

Test

Solution

5

Reaction

Biomolecule

Classification

A

No change

No change

Green

Yellow

No change

Sugar

B

No change

No change

No change

Pink

Gray

Starch

C

Pink

Purple

No change

Yellow

No change

Fat

D

No change

No change

No change

Pink

No change

Protein

E

Pink

No change

No change

Yellow

No change

Amino Acid

Observations and Questions

[5] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain sugars? If yes, which one? What information helped you to come to that decision? [6] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain protein? If yes, which one? What information helped you to come to that decision? [7] Using the laboratory tests in this experiment, would you be able to distinguish between a small molecule such as amino acid from a larger molecule such as a protein? Explain why or why not. [8] Using the laboratory tests in this experiment, would you be able to distinguish between a starch and a sugar such as glucose? Explain why or why not.
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